This article explores the issues surrounding the agroecological transition in the European wine industry, focusing on reducing pesticide use, developing organic certification and using genetic research in relation to resistant grape varieties. The study distinguishes between stakeholders from the wine industry, institutions and the agricultural research sector. The findings consistently identify the agroecological transition as a priority, particularly in terms of pesticide reduction. However, variations exist in the views of the surveyed stakeholders. French and Portuguese stakeholders emphasise the role of market and societal pressures as drivers of the transition, while Italian producers do not. Professionals in France and Portugal express doubts about achieving pesticide reduction through changes in practices, while others stress the importance of regulatory constraints. The research also highlights industry challenges such as decreased consumption due to health awareness and the need for social responsibility. Resistant grape varieties are seen as a viable solution, especially for the development of organic production, but market acceptability remains a significant hurdle. The study sheds light on stakeholder perspectives and challenges, thus contributing to a better understanding of priorities in the European wine industry's pursuit of sustainable practices.