This study assessed the subcritical water hydrolysis (SWH) of poultry feathers to recover amino acids. Experiments were conducted in a semi-continuous flow-through subcritical reactor (110mL), which was operated over a range of temperatures (210-250 °C) and water flow rates (5-15 mL min-1), combined through a 22 central composite design, at constant feed (10 g) and pressure (15 MPa). The results demonstrated that non-essential and essential amino acids were obtained from SWH of poultry feathers. The highest hydrolysis temperature resulted in the highest concentrations of valine, methionine, tryptophan, phenylalanine, isoleucine, leucine, and lysine. Otherwise, threonine, histidine, and arginine were obtained more effectively at lower temperatures. The response surface methodology was adopted to identify the best conditions for amino acid production, and it was possible to identify the ranges of temperatures and water flow rates to be used to recover specific amino acids. This study allowed concluding that SWH is a promising eco-friendly technology to recover amino acids from protein-rich wastes. All rights reserved, Elsevier.