Provided is a technique that promotes differentiation more efficiently without depending on initial conditions of stimulation when promoting differentiation of cells by a stimulation load. A method for producing differentiated cells or differentiated tissues, comprising: A culture step of culturing cells under conditions that produce a plurality of strain fields having different combinations of strains in a plurality of directions; a measurement step of non-invasively measuring an expression level of a gene related to differentiation of the cells in each of the strain fields in the culture step, And a conditioned culture step of conditioning and culturing the stimulation based on the results of the measuring step. None

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