[Problem] To provide a cell culture device that, when culturing adherent cells with a bag-shaped culture container, makes it possible to efficiently separate the adherent cells from the culturing surface and obtain a single cell. [Solution] A cell culture device (20) that uses a bag-shaped culture container (10) made from a soft package material to culture cells, said cell culture device (20) comprising: a stand (21) on which the culture container (10) is placed; a pressing member (24) which presses the culture container (10) placed on the stand (21) against the stand (21); and an impact imparting mechanism (25) that imparts an impact to the pressing member (24), wherein the pressing member (24) is provided so as to be inclinable with respect to the stand (21), and the impact imparting mechanism (25) imparts an impact to an end part of the pressing member (24) in a state where the culture...

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