Conventional single-use bioreactors have a drawback that the overall cost is increased when using a high-cost shaft or the like. The apparatus for sterilizing a bioreactor of the present invention is characterized by comprising a washing means for washing bioreactor accessories to be used after biochemical reaction use, which are installed in a single-use bag of a bioreactor, which is not used for biochemical reaction, and an ozone sterilization means for sterilizing the washed bioreactor accessories in the bag and the bag with ozone. Figure 5

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