Over the past few decades, green analytical chemistry has gradually gained prominence worldwide due to its economic, low toxicity, and environmentally friendly characteristics. The aim of this study was to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in situ detection of thiabendazole (TBZ). We take advantage of the reduction ability of office paper itself, achieving an in-situ reduction of AgNO3 to silver nanoparticles (Ag NPs) on paper without any additional chemical reducing agents. The investigation of the underlying mechanism reveals that aldehydes dissolved in paper play an important role in the reduction process. Reaction time, the concentration of AgNO3, and the heating temperature were optimized to achieve the best SERS performance. The prepared SERS paper had high SERS activity, good reproducibility, and stability, which was evaluated by the detection of model molecules R6G and MB. Finally, the as-prepared substrate was successfully applied to detect...

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