Recent increases in human activity and contamination of the marine environment have led to the advent of biosensors as sensitive, easy-to-operate, and rapid devices for water pollution screening. In the present study, a whole-cell electrochemical biosensor based on the native green microalgae Scenedesmus sp. MN738556 was developed, for the first time, to assess the biotoxicity of Cd2+ ions in freshwater. This electrochemical biosensor was constructed by immobilization of microalgae-BSA film on a glassy carbon electrode surface. The biosensor responses were based on chronoamperometric currents generated by alkaline phosphatase (ALP) activity. Since Cd2+ ions have an inhibition effect on ALP, the reduction in the general current can be used to determine the toxicity degree of these ions. The feasibility was evaluated and the application of the biosensor was optimized for parameters such as pH and cell density. The sensitivity of the biosensor was verified through the IC50 value...

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