Chrysanthellum indicum, an annual herb with yellow flowers, is used as a functional food in Asia and Africa for health purposes. In this study, we thoroughly investigated the characterization of phytochemicals within C. indicum's ethyl acetate, water, and methanolic extracts from both its bark and leaves. This was achieved through the utilization of the Folin Ciocalteu and aluminum trichloride assays. Moreover, the antioxidant properties of these extracts were comprehensively assessed employing microplate-based assays including ABTS, DPPH, CUPRAC, FRAP, MCA, and PBD, alongside online HPLC-based assays namely ABTS, DPPH, CUPRAC, and FRAP. Furthermore, the inhibitory activity against a range of enzymes, including AChE, BChE, tyrosinase, α-amylase, and α-glucosidase, was ascertained. The tested ethyl acetate extracts from bark and leaves had higher content of total phenolic and flavonoid compared to methanol and water extracts. Additionally, the bark extract exhibited superior antioxidant properties in the in vitro antioxidant assays. Meanwhile, in the...

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