To provide a composition that has a low thermal load in a concentration step, efficiently separates similar structures, and selectively concentrates DHA and HPA to provide a composition that contains both of the DHA and HPA in sufficient amounts. A process for preparing a composition containing ?-3 highly unsaturated fatty acid ester in which a docosahexaenoic acid ester and a Henopentaenoic acid ester are selectively concentrated, comprising treating a raw material containing the ?-3 highly unsaturated fatty acid ester with a lipase to concentrate the docosahexaenoic acid ester and the Henopentaenoic acid ester. None

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