An object of the present invention is to provide a novel material capable of immobilizing a biological substance. The present invention provides: A biological substance immobilization material in which a biological substance is immobilized to a modified pulp comprising a terminal carboxyl group-containing cellulose; a biological substance immobilization material in which the biological substance is immobilized to the modified pulp comprising a terminal carboxyl group-containing cellulose; a reaction step of reacting the modified pulp comprising the terminal carboxyl group-containing cellulose with the biological substance in the presence of a condensing agent, The method for producing the material; a method for adding the material to a sample collected from a subject and specifically binding the test substance in the sample to the biological substance immobilized on the material to confirm and/or quantify the presence or absence of the bound test substance, And combining and integrating the first and second layers with...

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