To provide a nucleic acid amplification method using a novel liquid feeding control mechanism.SOLUTION: A nucleic acid amplification method uses a nucleic acid amplification device comprising a chip for nucleic acid amplification and a substrate on which the chip for nucleic acid amplification can be mounted. The method comprises: a step 1 of mounting the chip for nucleic acid amplification on the substrate of the nucleic acid amplification device, the nucleic acid amplification device comprising first and second heaters, a mechanism for liquid feeding, first and second four-way valves, first and second two-way valves, a controller, first and second coupling parts, first to ninth channels, and the chip for nucleic acid amplification comprising first and second curve channels, an intermediate channel, a coupling part, and first and second coupling channels; a step 2 of coupling the coupling part on the chip for nucleic acid amplification with the first and second...

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