Provided is a method for performing a reverse transcription reaction using a nucleic acid polymerase having reverse transcription activity without using manganese. It has been found that modification of the amino acid corresponding to position 734 of the Thermus thermophilus-derived DNA polymerase enables efficient reverse transcription and reverse transcription reactions even in a reaction solution containing no MN 2+ (e.g., RT-PCR methods). The present invention provides methods, compositions, and the like using the modified nucleic acid polymerase. None

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