To allow for easily isolating membrane vesicles from a sample containing membrane vesicles.SOLUTION: A method of isolating membrane vesicles is provided, comprising: a complex forming step for bringing a sample into contact with a peptide or polymyxin B carried by a carrier to form a complex; and a dissociation step for bringing the complex into contact with a dissociation buffer to dissociate vesicles from the complex.SELECTED DRAWING: Figure 3

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