To provide a composite probiotic composition for alleviating overactive bladder-related symptoms.SOLUTION: A composite probiotic composition comprises Lactobacillus brevis strain GKJOY, Lactobacillus fermentum strain GKF3, Lactobacillus plantarum strain GKK1, and Pediococcus lactis strain GKA4. The composition is continuously administered to a subject for a given period; delays urination frequency effectively; and reduces bladder pressure for increased bladder capacity. Furthermore, the composition is used as an active ingredient for preparing various compositions.SELECTED DRAWING: Figure 4

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