[Object] to provide a cell frozen preservative that has a high cell survival rate after thawing, is excellent in safety, is low-cost, and allows easy acquisition of raw materials. [Solution] a cell frozen storage liquid is obtained by using physiological saline as a solvent and containing trehalose and collagen peptide as additives, preferably adding the collagen peptide to a trehalose solution containing trehalose at a concentration of 200 mm or more and 300 mm or less in the physiological saline, More preferably, the cell cryopreservation liquid is obtained by adding the collagen peptide at a ratio of 3 to 10 vol percent. Selected drawing: Figure 4

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