To provide a method for simply and accurately determining the presence of two or more kinds of specific oligonucleotide.SOLUTION: A determination method includes: a process of mixing a probe having a sequence complementary with two or more kinds of specific oligonucleotide and a measurement sample to obtain a mixture; a process of applying voltage between first solution containing the mixture and second solution which are partitioned by a lipid double membrane having a nanopore, and measuring the electric current intensity of electric current flowing between the first solution and the second solution over time to obtain electric current data over time; and a process of obtaining a plurality of interval time from the electric current data over time, performing a processing based on the central limit theorem regarding the plurality of interval time to obtain frequency distribution, identifying a content pattern derived from the two or more kinds of specific...

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