Disclosed is a method of testing an inhibitory effect of carbon dioxide on a spoilage causing ability of Shewanella putrefaciens, which includes activation, preparation of a bacterial suspension, preparation and treatment of samples, inoculation, transfer to packaging bag, modified atmosphere packaging, refrigeration and performance detection. After stored for 0-18 days, three inoculated samples are randomly selected and tested to evaluate inhibitory effects of different CO2 concentrations on the spoilage causing ability of Shewanella putrefaciens.

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