Sorbic acid-loaded chitosan/tripolyphosphate nanoparticles (SANs) have previously been shown to exert both antibacterial and antioxidant effects on Chinese sausage. In this study, the minimum inhibitory concentrations (MICs) of SANs against two Pseudomonas aeruginosa strains were determined. The blank control group (BC) served as the negative control, while the chitosan/tripolyphosphate nanoparticles (CTNs) group and free sorbic acid (SA) group served as the positive controls. Tests conducted under five different pH conditions (5/6/7/8/9) revealed that the SANs exhibited a good bacteriostatic effect against P. aeruginosa. Variations in the metabolism, cell membrane or cell wall integrity, and morphology of P. aeruginosa were measured to evaluate the effects of SANs on their intracellular and extracellular components. The MIC of SANs for the two P. aeruginosa strains was determined to be 150 μg/mL. SANs delayed the growth of P. aeruginosa and severely damaged both its inner and outer cell membranes. The heteromorphism of the...

Keywords
SORBIC ACID SEM CELL MEMBRANES CELL WALLS CHINESE SAUSAGES FOODS ANTIBACTERIAL ACTIVITY PSEUDOMONAS AERUGINOSA CHITOSAN ANTIOXIDATIVE ACTIVITY PH TRIPOLYPHOSPHATES METABOLISM MORPHOLOGY CELL WALLS PROCESSING NANOPARTICLES
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