Rapid detection methods require pre-enrichment culture in order to detect low levels of foodborne pathogens. To rapidly detect foodborne pathogens, enrichment culture processes could be replaced. Filtration and immunomagnetic separation methods have been identified to effectively concentrate and separate target pathogens from foods. In this study, a combination of filtration and immunomagnetic separation (IMS) has enabled the rapid and sensitive detection of foodborne pathogens. The pretreatment method, including separation and concentration procedures, increased sensitivity 10-100-fold. The sensitivity of a combination method using filtration and IMS to detect Escherichia coli O157:H7 and Salmonella enterica subsp. Enterica serovar Typhimurium was 10-101 CFU/10mL. In fresh-cut apples, IMS combined with filtration effectively improved the detection limit of real-time PCR to 2.70 × 101 CFU/g in E. coli O157:H7 and 1.80 × 102 CFU/g in Salmonella. The filtration simplified processing of large-volumes (250mL) and effectively concentrated pathogens while decreasing immunomagnetic beads...
Combination of filtration and immunomagnetic separation based on real-time PCR to detect foodborne pathogens in fresh-cut apple.
Se-Wook Oh, Department of Food and Nutrition, Kookmin University, Seoul, 136-702, South Korea. E-mail email@example.com
So-Young Lee, Jin-Hee Kim, Se-Wook Oh; Combination of filtration and immunomagnetic separation based on real-time PCR to detect foodborne pathogens in fresh-cut apple.. IFIS Food and Health Sciences Database 2023; doi:
Download citation file: