Effects of brassin on synthesis of anthocyanins in beans from beans.

In this experiment, the effect of brassin on the synthesis of anthocyanins in pods of beans was probed by studying the changes of the content of chromogenic substances in pods and the activities of key enzymes. Purple beans of four varieties including Zijiadou, Zilongjiadou, Dongfanghongjiadou, and Zididou were used as materials tested. Four mass concentrations of brassin (0.05, 0.10, 0.15, 0.20 mg/L) were sprayed three times at seedling stage, vine stage and flowering and pod setting stage. The results showed that spraying brassin could improve the content of chromogenic substances and enzyme activity in pods of beans. Among them, spraying 0.10 mg/L of brassin had the best effect, significantly increasing the contents of total flavonoids, proanthocyanidins, anthocyanins, and cyanins in four varieties, and the content of anthocyanins was the highest among the four varieties. Spraying 0.10 mg/L of brassica significantly increased the activities of dihydroflavonol reductase (DFR), anthocyanin synthase (ANS)...