To provide methods for determining whether aptamers that bind to target proteins can be created by G4 PAS method.SOLUTION: A method for determining whether an aptamer that binds to a desired target protein can be created by G4 PAS method comprises determining whether the target protein has a nuclear localization signal, or whether the target protein is a nuclear protein, and when a nuclear localization signal is present or when the protein is a nuclear protein, it is determined that the aptamer can be created by the G4 PAS method. The G4 PAS method comprises producing one or more aptamers having the same nucleotide sequence as one or more G4 structures in the promoter region of the gene encoding a target protein or in the promoter region of the gene of a transcriptional regulator that regulates transcription of the gene, and confirming that the produced aptamer binds to the target...
