Provided are a polypeptide having excellent 4-aminobenzoic acid hydroxylation activity and a method for using the polypeptide. In the amino acid sequence represented by SEQ ID NO: 2 or an amino acid sequence having at least 90 percent identity therewith, the polypeptide has 4-aminobenzoic acid hydroxylation activity, and the amino acid residue at position 39, position 43, position 83, position 106, position 180, position 266, position 346, position 363 or position 371, or at a position corresponding to position 39, position 43, position 83, position 106, position 180, position 266, position 346, position 363 or position 371 of the amino acid sequence represented by SEQ ID NO: 2 is the following amino acid: (a) position 39 or a position corresponding thereto: cysteine; (b) position 43 or a position corresponding thereto: leucine or histidine; (c) position 83 or a position corresponding thereto: valine, glycine or phenylalanine; (d) position 106 or a...

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