Double-stranded PNA probe

Provided are PNA probes of the type in which a quencher and a reporter are separated, which are capable of obtaining high fluorescence intensity when a target DNA and a PNA moiety are hybridized. A quencher part 20 comprising a quencher group 22, a DNA region 26, and a quencher linker 24 connecting the quencher group and the DNA region, and a light emitting group 12, a PNA region 16, And a reporter part 10 including a reporter linker 14 connecting the light-emitting group and the PNA region, and the quenching group and the light-emitting group are arranged on the same side when the DNA region and the PNA region are bound in a complementary manner, wherein the reporter linker does not contain two or more elements of oxygen in the main chain, Wherein the length of the double-stranded PNA probe is shorter than the reporter linker in terms of...