One embodiment of the present invention provides an online continuous biotoxicity measuring device comprising: a supply unit including a housing, a sample storage unit for storing a sample, and a culture medium storage unit for storing a culture medium for diluting the sample; a continuous dilution unit including a sample storage tank in which a sample supplied from the sample storage unit is stored, a culture medium storage tank in which a culture medium supplied from the culture medium storage unit is stored, and a plurality of dilution valves which are connected to a plurality of sample dispensing lines branched from the sample storage tank and a plurality of culture medium dispensing lines branched from the culture medium storage tank and are controlled such that the sample or culture medium are supplied at different dilution ratios; a chamber unit for accommodating an aquatic plant therein and continuously receiving a mixture...

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