Fate of foodborne pathogens during soaking and drying of walnuts.

Walnuts are among the most popular tree nuts that are soaked at home. Recipes for preparing soaked walnut kernels from online blogs (n = 71) and YouTube videos (n = 29) were reviewed to identify typical consumer handling practices that were then used to determine the fate of foodborne pathogens during soaking and subsequent drying of walnut kernels. Individual five-strain cocktails of rifampin-resistant Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella, grown on agar plates and diluted in water, were inoculated onto walnuts and then dried. Inoculated walnuts were added to sterile water at a ratio of 1:4 (w/v), held at 15, 18, or 22°C for up to 24 h, and then dried at 64°C for up to 24 h (for Salmonella-inoculated walnuts). Pathogen populations during soaking and drying were enumerated on tryptic soy agar with rifampin and on CHROM agar. Initial walnut moisture was ~4%,...