Surfactin could improve the quality of fresh noodles (FNs) during storage. The impacts of different levels of surfactin (0.0%, 0.2%, 0.3%, and 0.4%) on the quality and gluten network structure of FNs after storage (0d, 1d, 2d, and 3d) at 25 °C were investigated. The results showed that the addition of surfactin inhibited the tendency of hardness and tensile strength to decrease in FNs and improved their cooking properties. Size exclusion (SE-HPLC) and electrophoresis were employed to evaluate the strength of the gluten network, which indicated that surfactin increased the cross-linking of the gluten network during storage by promoting more disulfide bonds (SS). Surfactin increased the total contents of SDS-soluble polymers (SDS-P) and SDS-insoluble polymers (SDS-I) from 24.66% (control samples) to 33.32% (FNs with 0.4% surfactin) after 3 days of storage. The secondary structure of gluten network was determined using Fourier transform infrared (FTIR) spectroscopy, suggesting that surfactin increased α-helices...

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