Phenylpropanoid biosynthetic gene expression and nutrient uptake kinetics for enhanced rosmarinic acid production in suspension cultured cells of Halodule pinifolia.

This study investigated the effect of precursors (phenylalanine and tyrosine) and elicitors (methyl jasmonate and yeast) on enhanced rosmarinic acid (RA) biosynthesis, their transcript levels (HpPAL, HpC4H, Hp4CL, HpTAT and HpH PPR) and nutrient uptake kinetics from cell cultures of Halodule pinifolia (Miki) Hartog under in-vitro conditions. Different concentrations of precursors and elicitors were used, among which methyl jasmonate (20 μM) induced higher accumulation of RA (123.16 ± 3.30 mg g^-1 DW) followed by phenylalanine (57.086 ± 1.01 mg g^-1 DW), tyrosine (28.723 ± 1.55 mg g^-1 DW) and yeast elicitor (1.95 mg g^-1 DW). Further, the transcript levels of phenylpropanoid biosynthetic genes HpPAL, HpC4H and Hp4CL from MeJA treated cells were found to increase transiently, while TAT and HPPR did not express. It clearly conveyed that H. pinifola culture has undergone phenylpropanoid pathway for RA biosynthesis as compared to the control. Nutrient uptake kinetics determined...