Human milk oligosaccharides (HMOs) are a complex category of glycans present in high abundance in human milk, with its concentration and composition closely associated with infant health. Health benefits of HMOs include growth promotion of beneficial bacteria such as Bifidobacterium and inhibition of pathogenic agent adhesion to epithelial cell receptors. Ideally, the development of a robust, sensitive, and fast quantitative test method for key HMO species in human milk is desired for human clinical studies on HMOs, to further understand their benefits, related mechanisms and synthesis in relation to maternal phenotypes. Quantitative analysis of HMOs in human milk has been challenging due to the complex sample matrix, multiple components of HMOs and structurally similar isomers such as LNT and LNnT. In this study, a method has been successfully developed for the quantification of two pairs of neutral HMOs-namely the 2'-FL, 3-FL pair and LNT, LNnT pair, as well as a...

Keywords
LC-MS UPLC 2′-FUCOSYLLACTOSE 3′-FUCOSYLLACTOSE HUMAN MILK OLIGOSACCHARIDES
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