Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization.

Seed germination is the beginning of a new lifecycle, and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo. Although glutelin is a dominant storage protein in rice, its contribution to seed germination and its regulatory mechanisms are mostly unknown. Gibberellin (GA) and brassinosteroid (BR), two major growth-promoting phytohormones, also play positive roles in controlling seed germination. However, how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear. In the present study, biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth. Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity. Proteomic and qRT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo. Expression assays suggested...