The application of high-pressure technology (100-600 MPa, 20°C for up to 20min) for cell disruption and consequent extraction of proteins-including C-phycocyanin-from the cyanobacteria Arthrospira platensis (spirulina), was investigated. Wet spirulina biomass was suspended in three different aqueous systems (deionized water, phosphate buffer, 10% sodium chloride solution). During a-24 h post processing storage period at 20°C, the concentration of total soluble proteins and C-phycocyanin content and purity were measured. Color-spectrum and chroma analyses were also performed. The use of deionized water and phosphate buffer as processing/extraction media favoured the extraction process compared to the NaCl solution. Proteins extraction was significantly assisted by pressure. Equal/higher intensity than 400 MPa led to lower C-phycocyanin extraction yields, probably due to denaturation of proteins. High pressure conditions at 300 MPa for 10min-using deionized water or phosphate buffer as medium-were selected as optimum, leading to higher extraction yields and purity extracts within ~2 h after processing....

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