Acidification of milk destabilizes casein micelles (CMs) and results in network formation. This process is fundamental during the manufacturing of dairy gels such as cheese and yoghurts. Understanding the structural alterations of CMs during this process aids to predict the physicochemical and sensory properties of dairy gels. Herein we utilize direct Stochastic Optical Reconstruction Microscopy (dSTORM) to visualize individual hydrated CMs and characterize pH-dependent changes in CM size distributions. CMs were immobilized, fixed, labelled with caseins-specific primary antibodies, and imaged by dSTORM using Alexa Fluor 647 conjugated secondary antibodies. While antibodies specific to κ-and β-casein were used to stain CMs, only β-casein antibodies enabled reproducible imaging of micelles across the entire chosen pH window. Furthermore, CM's structural evolution was studied at acidic pH values representing the conditions during acid milk gel formation and at three elevated pH values. DSTORM imaging of casein aggregates at pH 4.5, below the isoelectric point...
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Journal Article|
October 20 2022
Super resolution microscopy imaging of pH induced changes in the microstructure of casein micelles.
I. K. Voets, Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry & Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, Netherlands. E-mail i.voets@tue.nl
Journal: Food Structure
Citation: Food Structure (2022) 30
DOI: 10.1016/j.foostr.2021.100231
Published: 2021
Citation
Foroutanparsa, S., Bruls, M., Tas, R. P., Maljaars, C. E. P., Voets, I. K.; Super resolution microscopy imaging of pH induced changes in the microstructure of casein micelles.. IFIS Food and Health Sciences Database 2022; doi:
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