Background. Understanding interactions between bacteria and fresh produce is of practical importance in the prevention and mitigation of foodborne and plant diseases. Microfluidic approaches, which usually operate at the same scale as produce-bacteria interactions, provide a platform for understanding interactions and detecting contamination. Scope and Approach. Here, we review microfluidic approaches suitable for use in fresh produce research and industry, classifying them based on their applications. The microfluidic devices were either used to unravel underlying mechanisms of fresh produce contamination or to develop detection tools for bacteria present on the produce. Three classes of microfluidic devices are discussed based on their common designs, as well as their applications in both fresh produce safety and sustainable production. Key Findings and Conclusions. The three classes of microfluidic devices are: (1) micropatterned surfaces (either with natural topography or simplified topography) used in the investigation of microbial attachment, infiltration, growth, and inactivation at the...

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