Minor flavonoids, such as isoquercitin, icariin and icariside II, have desired bioactivity and bioavailability. To enhance the production efficiency on converting rutin and epimedin C into minor flavonoids, a novel GH family 78 α-l-rhamnosidase PodoRha from the Paenibacillus odorifer was cloned and heterologous over-expressed in E. coli BL21 (DE3). PodoRha exhibited a high catalytic ability in cleaving outer α-1, 2-rhamnopyranosyl moieties of epimedin C and α-1, 6-rhamnopyranosyl of rutin. It displayed the highest activity at 45°C and pH 6.5, and has great thermostability at 40°C. Under the optimal transformation condition (40°C, pH 6.5, 1 h incubation), 10g/L of Rutin was 99.86% transformed into 6.95g/L of isoquercitin by PodoRha with a crude productivity of 6.95g. L-1. H-1; 1.964g/L icariin was detected after the epimedin C of the total Epimedium flavonoids (10g/L) were 99.98% converted by PodoRha. Moreover, by using a two-stage conversion system with a crude icariside...
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity.
Ning Zhang, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, National Engineering Lab for Biomass Chemical Utilization, Key and Open Lab of Forest Chemical Engineering, SFA, Key Lab of Biomass Energy Sources and Materials, Key Lab of Chemical Engineering of Forest Products, National Forestry and Grass and Administration, Nanjing, 210042, China. E-mail firstname.lastname@example.org
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Jingcong Xie, Jian Zhao, Ning Zhang, Hao Xu, Jing Yang, Jun Ye, Jianchun Jiang; Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity.. IFIS Food and Health Sciences Database 2022; doi:
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