An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (Î±-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E-) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E-fish contained 40-times more Î±-tocopherol (P <0.0001) and one fourth the malondialdehyde (P=0.0153). RNA was extracted from E+ and E-livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P...
Gene expression of CRAL_TRIO family proteins modulated by vitamin E deficiency in Zebrafish (Danio Rerio).
M. G. Traber, Linus Pauling Institute, 307 LPSC, Oregon State University, Corvallis, OR 97330, USA. E-mail email@example.com
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Watt, A. T., Head, B., Leonard, S. W., Tanguay, R. L., Traber, M. G.; Gene expression of CRAL_TRIO family proteins modulated by vitamin E deficiency in Zebrafish (Danio Rerio).. IFIS Food and Health Sciences Database 2022; doi:
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