Background. The Thermo ScientificTM SureTectTMSalmonella species PCR Assay utilizes SolarisTM reagents for performing PCR for the rapid and specific detection of Salmonella species in a broad range of foods and select environmental surfaces. Objective. The aims were to demonstrate the reproducibility of the Thermo Scientific SureTect Salmonella species PCR Assay in a collaborative study using a challenging matrix, cocoa powder, and to extend the scope of the method. Methods. In the collaborative study, the candidate method was compared to the US Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella reference method. The candidate method used two PCR thermocyclers, the Applied BiosystemsTM QuantStudioTM 5 Real-Time PCR instrument (QS5) and the Applied Biosystems 7500 Fast Real-Time PCR instrument (7500 Fast). Fourteen participants from nine laboratories located within the United States and Europe were solicited for the collaborative study, with 12 participants submitting...

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