In this study, polysaccharides from oyster mushrooms (Pleurotus ostreatus) were extracted using subcritical water (SW) from 120°C to 200°C. Polysaccharides obtained at the different conditions were used for chemical modification. Results. Showed that SW at 180°C recovered the highest amount of polysaccharides (20.35%) and sulfated polysaccharides (PS) obtained in this condition exhibited better anticoagulant activity than other conditions in intrinsic pathway. The degree of substitution of PS was 1.83. The molecular weight of polysaccharides decreased after chemical modification. Fourier transform infrared spectroscopy confirmed the success of modification process with the peaks of sulfite groups seen at 796cm-1, 1223cm-1, and 1379cm-1. In vitro anticoagulant activity showed that PS significantly improved the plasma clot form inhibition activity by intrinsic and extrinsic pathways compared to native polysaccharides. Moreover, the cytotoxicity of PS against two normal cell lines was relatively low. The findings in the present...

You do not currently have access to this content.