Validation of the Atlas^TM Campylobacter Detection assay: AOAC Performance Tested Method^SM 032101.

Background. Campylobacter spp. are a major causal agent for diarrheal illness in humans. Detection of Campylobacter spp. In food is critical to reduce foodborne illness, and to provide safe foods. Objective. The aim was to evaluate the Atlas Campylobacter Detection Assay for AOAC Performance Tested Methods^SM certification for detecting C. jejuni, C. coli, and C. lari in foods after 12 h enrichment. Method. The Atlas Campylobacter Detection Assay was compared to the ISO 10272-1:2017 reference culture method for chicken carcass rinse, turkey carcass sponge, raw ground poultry, raw ground pork, and ready-to-eat (RTE) meats. Inclusivity, exclusivity, product consistency, product stability, and robustness studies were also performed. An independent laboratory evaluated the performance of the Atlas Campylobacter Detection Assay method on chicken carcass rinse. Results. No significant differences were observed between the Atlas Campylobacter Detection Assay and the reference ISO method in spiked food matrixes. The Atlas Campylobacter...