Background. The testing of aflatoxins (AFs) in fresh and processed foods is highly in demand to comply with trade regulations. Consequently, commercial laboratories face huge AF sample loads in food consignments. Worldwide, there is a rising interest in implementing automation to increase sample throughput in AF analysis. Objective. This study sought to evaluate the performance of an automated cleanup and HPLC analysis system for determination of regulated AFs (B1, B2, G1, G2) in rice, flattened rice, sorghum, raw and processed peanut, almond, peanut butter, and wheat-based cookies. Methods. The samples were extracted with methanol-water (80:20), diluted with Triton X-100, and subjected to automated analysis, where the cleanup step through immunoaffinity column (IAC) and HPLC-fluorescence analyses (involving postcolumn bromination-derivatization) were performed in 10 and 11min, respectively. The method was validated in all test matrices at the LOQ and higher levels. The method performance was also evaluated against a conventional workflow where...

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