Phytosterol (PS) has many excellent physiological activities, but the application of phytosterol in food industry is widely restricted due to low solubility and bioaccessibility. To provide a water-soluble phytosterol, okara protein (OP) was used as raw material to prepare phytosterol nanoparticles by anti-solvent method combined with dynamic high-pressure microfluidization, and physicochemical property were characterized. Dynamic high-pressure microfluidization can significantly reduce the size of OP-PS particles, and significantly improve the encapsulation efficiency of PS. After dynamic high-pressure microfluidization for 3 times at 120 MPa, the particle size of OP-PS particles can reach 139.28 nm with good re-dispersibility, and the encapsulation efficiency of PS is up to 98.63%, which can effectively improve the water-solubility and bioavailability of PS. The stability of OP-PS particles was evaluated. After 50 days of storage at room temperature, the encapsulation efficiency of PS was still about 66.90%, and the thermal stability was obviously better than that of...

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