Î•-Polylysine (Îµ-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (Îµ-PL) against food poisoning pathogens detected in chicken (Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031-1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to Îµ-PL depended on the time and concentration, and 1/2Ã—MIC of Îµ-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (Salmonella, Listeria, E. coli, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2Ã—MIC of Îµ-PL, respectively. Additionally, when observed under a CLSM,...
Antimicrobial and antibiofilm effect of ε-polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in tryptic soy broth and chicken juice.
Hyun-Dong Paik, Department of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, Korea. Tel. +82-2-2049-6011. E-mail email@example.com
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Do-Un Lee, Yeong Jin Park, Hwan Hee Yu, Suk-Chae Jung, Jung-Hee Park, Dae-Hee Lee, Na-Kyoung Lee, Hyun-Dong Paik; Antimicrobial and antibiofilm effect of ε-polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in tryptic soy broth and chicken juice.. IFIS Food and Health Sciences Database 2022; doi:
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