An immunized mouse phage display scFv library with a capacity of 3.34 Ã— 109 CFU/mL was constructed and used for screening of recombinant anti-ciprofloxacin single-chain antibody for the detection of ciprofloxacin (CIP) in animal-derived food. After four rounds of bio-panning, 25 positives were isolated and identified successfully. The highest positive scFv-22 was expressed in E. coli BL21. Then, its recognition mechanisms were studied using the molecular docking method. The result showed the amino acid residue Val160 was the key residue for the binding of scFv to CIP. Based on the results of virtual mutation, the scFv antibody was evolved by directional mutagenesis of contact amino acid residue Val160 to Ser. After the expression and purification, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) based on the parental and mutant scFv was established for CIP, respectively. The IC50 value of the assay established with the ScFv mutant was 1.58 ng/mL, while...
Preparation and directed evolution of anti-ciprofloxacin ScFv for immunoassay in animal-derived food.
Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, 116#Huayuan Road, Zhengzhou 450002, China. E-mail firstname.lastname@example.org
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Fangyu Wang, Ning Li, Yunshang Zhang, Xuefeng Sun, Man Hu, Yali Zhao, Jianming Fan; Preparation and directed evolution of anti-ciprofloxacin ScFv for immunoassay in animal-derived food.. IFIS Food and Health Sciences Database 2022; doi:
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