The response surface methodology (RSM) and central composite design (CCD) technique were used to optimize the three key process parameters (i.e., pressure, temperature and holding time) of the high-hydrostatic-pressure (HHP) processing either standalone or combined with moderate thermal processing to modulate molecular structures of Î²-lactoglobulin (Î²-Lg) and Î±-lactalbumin (Î±-La) with reduced human IgE-reactivity. The RSM model derived for HHP-induced molecular changes of Î²-Lg determined immunochemically showed that temperature (temp), pressure (p2) and the interaction between temperature and time (t) had statistically significant effects (p < 0.05). The optimal condition defined as minimum (Î²-Lg specific) IgG-binding derived from the model was 505 MPa at 56 Â°C with a holding time of 102 min (R2 of 0.81 and p-value of 0.01). The validation carried at the optimal condition and its surrounding region showed that the model to be underestimating the Î²-Lg structure modification. The molecular...
A response surface methodology (RSM) approach for optimizing the attenuation of human IgE-reactivity to β-lactoglobulin (β-Lg) by hydrostatic high pressure processing.
N. A. Lee, School of Chemical Engineering, University of New South Wales, Syndey, NSW 2052, Australia. Tel. +61-2-93854363. E-mail email@example.com
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Xin Sun, Chua, J. V., Quynh Anh Le, Trujillo, F. J., Mi-Hwa Oh, Campbell, D. E., Mehr, S., Lee, N. A.; A response surface methodology (RSM) approach for optimizing the attenuation of human IgE-reactivity to β-lactoglobulin (β-Lg) by hydrostatic high pressure processing.. IFIS Food and Health Sciences Database 2022; doi:
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