Metabolic engineering substantially aims at the development of more efficient, robust and industrially competitive microbial strains for the potential applications in food, fermentation and pharmaceutical industries. An efficient lab scale bioprocess was developed for high level fermentative production of L-alanine using metabolically engineered Pediococcus acidilactici BD16 (alaD+). Computational biology tools assisted the designing of a synthetic alaD gene cassette, which was further cloned in shuttle vector pLES003 and expressed using an auto-inducible P289 promoter. Further, L-alanine production in the recombinant P. acidilactici BD16 (alaD+) strain was carried out using fed-batch fermentation under oxygen depression conditions, which significantly enhanced L-alanine levels. The recombinant strain expressing the synthetic alaD gene produced 229.12 g/L of L-alanine after 42 h of fed-batch fermentation, which is the second highest microbial L-alanine titer reported so far. After extraction and crystallization, 95% crystal L-alanine (217.54 g/L) was recovered from the culture broth...
Metabolic engineering of Pediococcus acidilactici BD16 for heterologous expression of synthetic alaD gene cassette and L-alanine production in the recombinant strain using fed-batch fermentation.
Baljinder Kaur, Systems Biology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147002, India. E-mail firstname.lastname@example.org
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Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Baljinder Kaur, Ajaz Ahmad; Metabolic engineering of Pediococcus acidilactici BD16 for heterologous expression of synthetic alaD gene cassette and L-alanine production in the recombinant strain using fed-batch fermentation.. IFIS Food and Health Sciences Database 2022; doi:
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