The research aims to extract nutrients and bioactive compounds from Spirulina using a non-toxic, environmentally friendly and efficient method-Pressurized Liquid Extraction (PLE). In this work, Response Surface Methodology (RSM)-Central Composite Design (CCD) was used to evaluate and optimize the extraction time (5-15 min), temperature (20-60°C) and pH (4-10) during PLE extraction (103.4 bars). The multi-factor optimization results of the RSM-CCD showed that under the pressure of 103.4 bars, the optimal conditions to recover the highest content of bioactive compounds were 10 min, 40°C and pH 4. Furthermore, the compounds and antioxidant capacity of PLE and non-pressurized extraction extracts were compared. The results showed that under the optimal extraction conditions (10 min, 40°C and pH 4), PLE significantly improved the antioxidant capacity (2870.5±153.6 µM TE), protein yield (46.8±3.1%), chlorophyll a (1.46±0.04 mg/g), carotenoids (0.12±0.01 mg/g), total polyphenols (11.49±0.04 mg/g) and carbohydrates content (78.42±1.40 mg/g) of the extracts compared with non-pressurized extraction...

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