Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X1) and enzyme usage (X2) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and% yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X1 at 8.63 h and X2 at 0.93% provided the highest values of DH and% yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to...
Study of the lipolysis effect of nanoliposome-encapsulated Ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach.
Kiattawee Choowongkomon, Interdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok 10900, Thailand. Tel. +66-2-562-5444, ext. 2046. E-mail firstname.lastname@example.org
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Sucheewin Krobthong, Yodying Yingchutrakul, Wonnop Visessanguan, Thanisorn Mahatnirunkul, Pawitrabhorn Samutrtai, Chartchai Chaichana, Phakorn Papan, Kiattawee Choowongkomon; Study of the lipolysis effect of nanoliposome-encapsulated Ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach.. IFIS Food and Health Sciences Database 2022; doi:
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