The antiadipogenic activity of conjugated linoleic acids (CLA) in the form of phosphatidylcholine-bound (CLA-PC) or free fatty acids (FFA; CLA-FFA) was evaluated using 3T3-L1 adipocytes. Phosphatidylcholine from soya (soy-PC) was used as the comparison of PC form. Both the lipid accumulation and activity of glycerol-3-phosphate dehydrogenase were measured to determine lipogenesis, whereas the glycerol content was measured to evaluate lipolysis. The CLA uptake also measured to find out the utilization of CLA by the cells. As a results, lipid accumulation in 3T3-L1 adipocytes was inhibited in a dose-dependent manner following treatment with CLA-PC (50-400 Î¼M). Both CLA-PC and soy-PC significantly suppressed lipid accumulation compared with CLA-FFA, even though the amount of CLA in CLA-PC was a half than CLA-FFA. The CLA uptake of PC form was superior to FFA form, however, no difference was noted between CLA-PC and soy-PC. These forms exerted their antiadipogenic activity via the suppression of lipogenesis,...
Antiadipogenic effects of different molecular forms of conjugated linoleic acid on 3T3-L1 cells: comparison between free fatty acid and phosphatidylcholine forms.
Y. Yamamoto, Faculty of Bioresource Science, Prefectural University of Hiroshima, 5562 Nanatsuka-cho, Shobara, Hiroshima, 727-0023, Japan. E-mail email@example.com
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Fauziah, R. R., Chin, R., Ogita, S., Yoshino, T., Yamamoto, Y.; Antiadipogenic effects of different molecular forms of conjugated linoleic acid on 3T3-L1 cells: comparison between free fatty acid and phosphatidylcholine forms.. IFIS Food and Health Sciences Database 2022; doi:
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