Gamma-aminobutyric acid (GABA) plays a key role as an inhibitory neurotransmitter in the mammalian sympathetic nervous system and has other health benefits. Molecular characterization, genome analysis, and optimization were investigated to improve GABA production of a selected strain of lactic acid bacteria. Eleven isolates from plant materials were screened for GABA productivity and were identified based on phenotypic and genotypic characteristics. The most potent strain was chosen for genome analysis and GABA production optimization using the response surface methodology (RSM). Each of the two strains was closely related to Lactobacillus plantarum, Lactobacillus brevis, Weissella cibaria, Leuconostoc pseudomesenteroides while each strain was similar to Lactobacillus pentosus, Enterococcus lactis, and Leuconostoc mesenteroides. They produced GABA ranging from 0.036 Â± 0.000 to 17.315 Â± 0.171 g/L at 72 h-cultivation. Among them, the most potent strain, SL9-6, showed the highest GABA production (17.315 g/L) when cultivated with 10%...
Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials.
Somboon Tanasupawat, Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Bangkok 10330, Thailand. Tel. +66-2-218-8376. Fax +66-2-254-5195. E-mail Somboon.T@chula.ac.th
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Sukanya Phuengjayaem, Nattakorn Kuncharoen, Auttaporn Booncharoen, Boonsri Ongpipattanakul, Somboon Tanasupawat; Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials.. IFIS Food and Health Sciences Database 2022; doi:
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