Sulphur amino acids (SAA) are essential for multiple physiological/metabolic processes, with the ratio of dietary methionine: cysteine (Met: Cys) being an important contributor to pro-inflammatory responses, including TNF-Î± activity. The current study was designed to determine the effect an altered dietary SAA ratio, and the resulting reliance on the transsulfuration pathway to supply Cys, will have on the inflammatory response. In the present study, 100 Âµg/kg of an intraperitoneal (IP) injection of lipopolysaccharide (LPS) was used as a model for systemic inflammation. Male Wistar rats were randomized to one of two amino acid-defined diets, (100Met:0Cys or 50Met:50Cys) and subdivided to receive either IP LPS or saline injections. LPS significantly increased total plasma Cys, homocysteine (Hcy) and glutathione (GSH) 240 min post-IP injection in rats fed a 50Met:50Cys ratio compared to other treatments. The TNF-Î± area under the curve for LPS-treated rats consuming a dietary 50Met:50Cys ratio was significantly higher (...
Alteration of the dietary methionine: cysteine ratio modulates the inflammatory response to an inter-peritoneal injection of lipopolysaccharide in wistar rats.
J. D. House, 208A Human Ecology, Winnipeg, Man. R3T 2N2, Canada. Tel. +12044746837. Fax +12044747593. E-mail firstname.lastname@example.org
- Views Icon Views
- Share Icon Share
- Search Site
Medina, G., Nosworthy, M. G., Petkau, J. C., Blewett, H., Li, S., House, J. D.; Alteration of the dietary methionine: cysteine ratio modulates the inflammatory response to an inter-peritoneal injection of lipopolysaccharide in wistar rats.. IFIS Food and Health Sciences Database 2022; doi:
Download citation file: