The application of salting out assisted liquid-liquid extraction for the rapid and simple simultaneous determination of pyrrolizidine alkaloids and related N-oxide in honey and pollen was evaluated. The determination of PAs and PANOs was obtained by ultra high performance liquid chromatography coupled with tandem mass spectrometry. The critical parameters (pH of the aqueous solution, extraction solvent, type and amount of salting-out agents) affecting SALLE performance were carefully studied and optimized using a chemometric approach. Under the optimal experimental conditions, the developed procedure showed excellent extraction efficiency (82-104%) and recoveries (82-113%) for all target analytes, except for Intermedine N-oxide, at three concentration levels (0.25, 2.5 and 25 μg kg-1 and 2.5, 25 and 250 μg kg-1 for honey and pollen samples, respectively), and satisfactory intra-day (<10) and inter-day (<13) precisions. The proposed method showed negligible matrix effect for both studied matrices and method quantification limits at very low levels...
Skip Nav Destination
Journal Article|
March 10 2022
Salting-out Assisted Liquid-Liquid Extraction for the rapid and simple simultaneous analysis of pyrrolizidine alkaloids and related N-oxides in honey and pollen.
R. Celano, Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, Fisciano, Salerno, 84084, Italy. E-mail rcelano@unisa.it
Journal: Journal of Food Composition and Analysis
Citation: Journal of Food Composition and Analysis (2022) 108
DOI: 10.1016/j.jfca.2022.104457
Published: 2022
Citation
Rizzo, S., Celano, R., Campone, L., Rastrelli, L., Piccinelli, A. L.; Salting-out Assisted Liquid-Liquid Extraction for the rapid and simple simultaneous analysis of pyrrolizidine alkaloids and related N-oxides in honey and pollen.. IFIS Food and Health Sciences Database 2022; doi:
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your Institution
Analyse Trends
Explore publication trends in the sciences of food and health.
Discover and compare the use of keywords over time.
Find global trends in research through publication categories.