To realize the safe production of D-tagatose, a strain can produce L-arabinose isomerase was obtained by screening and identified as Lactobacillus brevis D-tag1. The carbon source, nitrogen source, fermentation temperature and initial pH of fermentation were optimized to improve the enzyme production capacity. The results showed that at the optimal conditions of initial pH 6.0. Fermentation temperature 37°C and fermentation time 24 h, the enzyme activity could reach to 6.8 U/mL. Nearly 2.3 times of that before optimization. Using 9.0% D-galactose as substrate, the conversion of D-galactose to D-tagatose was 43% to 44% at pH 7.0, 55°C, after 48 h of whole cell catalytic reaction.

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